By Allen I. Laskin
Advances in utilized Microbiology deals in depth experiences of the most recent innovations and discoveries during this speedily relocating box. The editors are famous specialists and the layout is complete and instructive.
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Technological know-how schooling is experiencing a revitalization, because it is well-known that technological know-how may be available to everybody, not only society’s destiny scientists. a method to make the examine of technology extra sizeable to the non-major is to require a laboratory part for all technological know-how classes. the topic of utilized botany with its emphasis at the functional facets of plant technological know-how, the authors think, could be beautiful to the non-major because it exemplifies how a simple technology should be utilized to challenge fixing.
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Additional resources for Advances in Applied Microbiology: Volume 54
This approach has been particularly useful in dry environments, and success by LUBILOSA and others in low-humidity environments with oils has revolutionized thinking on the previously widely accepted concept that high humidity is required for fungal insect control. Oils have been used with chemical insecticides, so there is a considerable body of literature on oils appropriate to application to plants. These and other oils, such as kerosene or vegetable oils, were adopted by the LUBILOSA project to develop Ma-ac for locust control in the deserts of Africa.
Inoculation is usually by repeating syringe with the needle inserted directly into the bag of autoclaved rice or other substrate. The bag may be wiped with alcohol before puncture and the hole closed afterward with tape. In large operations, the inoculum is mycelium in the log phase of growth produced in submerged culture. The mycelium initiates growth and sporulation considerably faster than inoculating with dormant conidia. In some cases, there is immediate massaging of the bag to evenly distribute the inoculum and to further ensure that the substrate is not aggregated.
We used this strategy to develop the first genetically improved entomopathogenic fungus (St. , 1996). Additional copies of the gene encoding the cuticle-regulated Pr1a protease were inserted into the genome of Ma-an ARSEF #1080 under the control of a constitutive promoter such that the gene was constitutively expressed. In contrast to the wild type, the transgenic strains continued to produce Pr1 in the hemocoel of Manduca sexta caterpillars following penetration of the cuticle. This caused extensive melanization in the body cavity and reduced time to death by 25%.